Phosphoproteomics of hES
Phosphoproteomics of hES
Human embyronic stem cells (hES cells) were first cultured here at the University of Wisconsin by Jamie Thomson and are of great interest to the scientific and medical community for as they have the potential to differentiate into any type of human cell (pluripotency). However, the mechanisms that govern the maintenance of pluripotency and differentiation are not clear. We are applying ETD technology to the the phosphoproteome of hES cells to elucidate the pathways involved in these mechanisms. ETD is perfectly suited for this application, in contrast to other fragmentation methods, because it does not cleave phosphorlyation sites from the peptide backbone. This allows for identification of phosphorylation at the amino acid level. Site specific phosphoyrlation can then be compared for cells in different states of differentiation to identify phosphoyrlation events that may be essential in these mechanisms.
Human embyronic stem cells (hES cells) were first cultured here at the University of Wisconsin by Jamie Thomson and are of great interest to the scientific and medical community for as they have the potential to differentiate into any type of human cell (pluripotency). However, the mechanisms that govern the maintenance of pluripotency and differentiation are not clear. We are applying ETD technology to the the phosphoproteome of hES cells to elucidate the pathways involved in these mechanisms. ETD is perfectly suited for this application, in contrast to other fragmentation methods, because it does not cleave phosphorlyation sites from the peptide backbone. This allows for identification of phosphorylation at the amino acid level. Site specific phosphoyrlation can then be compared for cells in different states of differentiation to identify phosphoyrlation events that may be essential in these mechanisms.