Chemistry 638: Introduction to Mass Spectrometry
Spring 2008, 1 credit, Thursdays 9:55 am, room 2311, Chemistry
Instructors: Martha M. Vestling, Amy C. Harms
Mass spectrometry has evolved into one of the leading technologies for
biological and chemical research. It is widely used in genomics, proteomics,
drug discovery, forensics, polymer characterization, and combinatorial chemistry.
This course is an opportunity for you to learn more about this exciting technology.
Topics to be covered:
xxxxxbasic vocabulary of mass spectrometry
xxxxxionization options and instrument types
xxxxxbasic sample handling, common cantaminants
xxxxxdatabases
xxxxxfragmentation and sequencing
xxxxxseparations before mass spectrometry: LCMS, GCMS
xxxxxpeptides and proteins, post-translational modifications
xxxxxwhen to use stable isotopes
xxxxxmetabolite identification
xxxxxquantitative analysis
Lectures will mix the basics of mass spectrometry with examples of sophisticated
applications. Grades will be based on attendance and a short paper.
Text: "The Expanding Role of Mass Spectrometry in Biotechnology," 2nd
ed.,
Gary R. Siuzdak, MCC Press, San Diego, 2006.
Lab 1 Lab 2
copyright © 2008 CIC Mass Spectrometry Facility