Chemistry 638: Introduction to Mass Spectrometry
Spring 2009, 1 credit, Thursday 9:55 am, Room 2311 Chemistry Instructors: Martha M. Vestling, Amy C. Harms
Mass spectrometry has evolved into one of the leading technologies for biological and chemical research. It is widely used in genomics, proteomics, drug discovery, forensics, polymer characterization, and combinatorial chemistry.
Topics to be covered:
- basic vocabulary of mass spectrometry
- ionization options and instrument types
- basic sample handling, common contaminants
- databases
- fragmentation and sequencing
- separations before mass spectrometry: LCMS, GCMS
- peptides and proteins, post-translational modifications
- when to use stable isotopes
- metabolite identifications
- quantitative analysis
Lectures will mix the basics of mass spectrometry with examples of sophisticated applications. Grades will be based on attendance and a short paper.
Text: "The Expanding Role of Mass Spectrometry in Biotechnology," 2nd ed., Gary R. Siuzdak, MCC Press, San Diego, 2006.
Lab 1 Lab 2