 Central Michigan University (B.S.)
University of Florida (Ph.D.)
University of Virginia (Postdoctoral Fellow)
Room: 3233
Phone: 608-263-1718
Email: jcoon@chem.wisc.edu
Position: Assistant Professor
Swaney DL, Thomson JA, Coon JJ. Human Embryonic Stem Cell Phosphoproteome Revealed by Electron Transfer Dissociation Tandem Mass Spectrometry. Proceedings of the National Academy of Sciences of the United States of America, 2009, in press. McAlister GC, Berggren WT, Griep-Raming J, Horning S, Makarov A, Phanstiel D, Stafford GC, Swaney DL, Syka JEP, Coon JJ. A Proteomics Grade ETD-enabled Linear Ion Trap-Orbitrap Hybrid Mass Spectrometer. Journal of Proteome Research, 2008, 7 (8), 6388-6394. Hubler, SL, Jue A, Keith J, McAlister GC, Cracium G, Coon JJ. Valence Parity Renders z-type ions Chemically Distinct. Journal of the American Chemical Society, 2008. Phanstiel D, Brumbaugh J, Berggren WT, Feng X, Levenstein ME, Thomson JA, Coon JJ. Mass Spectrometry Identifies and Quantifies 74 Unique Histone H4 Isoforms in Differentiating Human Embryonic Stem Cells. Proceedings of the National Academy of Sciences of the United States of America, 2008, 104, 11, 4093-4098. Phanstiel D, Brumbaugh J, Berggren WT, Feng X, Levenstein ME, Thomson JA, Coon JJ. Mass Spectrometry Identifies and Quantifies 74 Unique Histone H4 Isoforms in Differentiating Human Embryonic Stem Cells. Proceedings of the National Academy of Sciences of the United States of America, 2008, 105, 11, 4093-4098. Brumbaugh J, Phanstiel P, Coon JJ. Unraveling the Histone's Potential: a Proteomics Perspective. Epigenetics, 2008, 3:5, 1-4. Good DM, McAlister GC, Phanstiel D, Coon JJ. Performance Evaluation of Electron Transfer Dissociation. Molecular and Cellular Proteomics, 2007, 6, 11, 1942-1951. Khidekel N, Ficarro SB, Clark PM, Swaney DL, Rexach JE, Coon JJ, Peters EC, Hsieh-Wilson LC. Probing the Dynamics of O-GlcNAc Glycosylation in the Brain using Quantitative Proteomics. Nature Chemical Biology, 2007, 3, 6, 339-348. McAlister GC, Phanstiel D, Good DM, Berggren WT, Coon JJ. Implementation of Electron Transfer Dissociation on a Hybrid Linear Ion Trap-Orbitrap Mass Spectrometer. Analytical Chemistry, 2007, 79, 10, 3525-3534. Swaney DL, McAlister GC, Wirtala M, Schwartz JC, Syka JEP, Coon JJ. A supplemental activation method for high efficiency electron transfer dissociation of doubly protonated peptide precursors. Analytical Chemistry, 2007, 79, 2, 477-485.
| Research Description
Research Emphasis: Bioanalytical Chemistry, Mass Spectrometry & Proteomics
Overview. My research group has the overarching goal of catalyzing evolution in the rapidly developing field of proteomics and to use these technologies to address fundamental problems in developmental biology. With emphasis on ion chemistry and instrumentation, we seek to develop and apply new enabling mass spectrometry-based (MS) proteomic technologies. These cutting-edge tools allow us to examine, with unprecedented chemical detail and sensitivity, the molecular events that commit human embryonic stem cells (hES cells) to exit the pluripotent state. Here we are focused on both intracellular signaling and the epigenetic regulation of pluripotency. For the former we ask which branches of the FGF signaling pathway are active in hES cells and which proteins/networks are phosphorylated upon differentiation. Epigenetics is believed to play a critical role in the establishment and maintenance of pluripotency; thus, we have also aimed our new technologies at interpreting the epigenetic codes and monitoring how these messages change during hES cell differentiation.
Active projects. Research projects in the Coon lab include: (1) instrumentation development, (2) data analysis software design, (3) fundamental ion chemistry studies, and (4) biological applications of the technology. Biological applications include global identification of protein post-translational modification (specifically phosphorylation), quantitative analysis of protein phosphorylation (i.e., comparative analysis of two cellular states), and cancer biomarker discovery.
Last Updated: 28 January 2009
Pittsburgh Conference Achievement Award, 2010 Ken Standing Award, 2009 National Science Foundation CAREER Award Eli Lilly and Company Young Investigator Award
Beckman Young Investigator Award American Society of Mass Spectrometry Research Award Named one of "Tomorrow's PIs" by Genome Technology magazine Ruth L. Kirchstein Individual National Research Service Award (NIH-NRSA)
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