All Department Publications

Found 13 results
Author Title Type [ Year(Asc)]
Filters: Author is Saecker, Ruth M.  [Clear All Filters]
2011
Saecker RM, Record MT, Dehaseth PL. Mechanism of bacterial transcription initiation: RNA polymerase - promoter binding, isomerization to initiation-competent open complexes, and initiation of RNA synthesis. J Mol Biol. 2011;412(5):754-71.
Koh J, Shkel I, Saecker RM, Record MT. Nonspecific DNA Binding and Bending by HU(alpha beta): Interfaces of the Three Binding Modes Characterized by Salt-Dependent Thermodynamics. Journal of Molecular Biology. 2011;410:241-267.
2010
Gries TJ, Kontur WS, Capp MW, Saecker RM, Record MT. One-step DNA melting in the RNA polymerase cleft opens the initiation bubble to form an unstable open complex. Proceedings of the National Academy of Sciences of the United States of America. 2010;107:10418-10423.
Kontur WS, Capp MW, Gries TJ, Saecker RM, Record MT. Probing DNA Binding, DNA Opening, and Assembly of a Downstream Clamp/Jaw in Escherichia coil RNA Polymerase-lambda P-R Promoter Complexes Using Salt and the Physiological Anion Glutamate. Biochemistry. 2010;49:4361-4373.
2008
Meulen KVA, Saecker RM, Record MT. Formation of a wrapped DNA-protein interface: Experimental characterization and analysis of the large contributions of ions and water to the thermodynamics of binding IHF to H ' DNA. Journal of Molecular Biology. 2008;377:9-27.
Kontur WS, Saecker RM, Capp MW, Record MT. Late steps in the formation of E-coli RNA Polymerase-lambda P-R promoter open complexes: Characterization of conformational changes by rapid [Perturbant] upshift experiments. Journal of Molecular Biology. 2008;376:1034-1047.
2007
Davis CA, Bingman CA, Landick R, Record MT, Saecker RM. Real-time footprinting of DNA in the first kinetically significant intermediate in open complex formation by Escherichia coli RNA polymerase. Proceedings of the National Academy of Sciences of the United States of America. 2007;104:7833-7838.
2006
Kontur WS, Saecker RM, Davis CA, Capp MW, Record MT. Solute probes of conformational change in open complex formation by E coli RNA polymerase at the lambdaPR promoter: evidence for unmasking of the active site in the isomerization step and for largescale coupled folding in the subsequent conversion to RPo. Biochemistry. 2006;45(7):2161-77.
2005
Hong J, Capp MW, Saecker RM, Record MT. Use of urea and glycine betaine to quantify coupled folding and probe the burial of DNA phosphates in lac repressor-lac operator binding. Biochemistry. 2005;44(51):16896-911.
2004
Hong J, Capp MW, Anderson CF, Saecker RM, Felitsky DJ, Anderson MW, Record MT. Preferential interactions of glycine betaine and of urea with DNA: implications for DNA hydration and for effects of these solutes on DNA stability. Biochemistry. 2004;43(46):14744-58.
2003
Saecker RM, Tsodikov OV, Capp MW, Record MT. Rapid quench mixing to quantify kinetics of steps in association of Escherichia coli RNA polymerase with promoter DNA. Methods Enzymol. 2003;370:535-46.
2002
Saecker RM, Tsodikov OV, McQuade KL, Schlax PE, Capp MW, Record MT. Kinetic studies and structural models of the association of E. coli sigma(70) RNA polymerase with the lambdaP(R) promoter: large scale conformational changes in forming the kinetically significant intermediates. J Mol Biol. 2002;319(3):649-71.
Saecker RM, Record MT. Protein surface salt bridges and paths for DNA wrapping. Curr Opin Struct Biol. 2002;12(3):311-9.

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