| Title | Bovine Pancreatic Ribonuclease: Fifty Years of the First Enzymatic Reaction Mechanism |
| Publication Type | Journal Article |
| Year of Publication | 2011 |
| Authors | Cuchillo, CM, Victoria Nogues, M, Raines, RT |
| Journal | Biochemistry |
| Volume | 50 |
| Pagination | 7835-7841 |
| Date Published | Sep |
| Accession Number | WOS:000294791100001 |
| Keywords | acetoacetate decarboxylase, acid, active-site, AKYNE CA, 1979, V101, P3951, AVIN A, 1966, V101, PC14, BARNARD EA, 1969, V38, P677, Biochemistry & Molecular Biology, BLACKBURN P, 1982, V15, P317, BLAKE CCF, 1967, V167, P378, catalytic mechanism, CUCHILLO CM, 1993, V333, P207, Cuchillo CM, 2002, V11, P117, cytidine 2'-3'-phosphate, EFTINK MR, 1987, P333, FERSHT A, 1977,, fersht a, 1998, FINDLAY D, 1961, V190, P781, FINDLAY D, 1962, V85, P152, Fisher BM, 1998, V273, P34134, FREY PA, 1971, V93, P7266, Hengge AC, 2000, V28, P119, HERRIES DG, 1962, V85, P127, histidine-residues, ionization constant, JACKSON DY, 1994, V266, P243, KARTHA G, 1967, V213, P862, KOKESH FC, 1971, V93, P7270, Kunitz M, 1939, V90, P112, LCARDAYRE SB, 1994, V33, P6031, LCARDAYRE SB, 1995, V252, P328, magnetic-resonance, MARKHAM R, 1952, V52, P552, MARKLEY JL, 1975, V14, P3546, MESSMORE JM, 1995, V117, P8057, Moussaoui M, 1998, V273, P25565, Moussaoui M, 2007, V16, P99, Nnis ea, 1966, v88, p3431, Park C, 2001, V40, P4949, Phosphate-binding subsite, reporter group, ROBERTS GCK, 1969, V62, P1151, ROSS CA, 1962, V85, P145, RUSHIZKY GW, 1961, V236, P2732, SMYTH DG, 1963, V238, P227, THOMPSON JE, 1994, V116, P5467, USHER DA, 1970, V228, P663, Vlin tm, 2002,, VOET D, 2004,, WALSH C, 1979,, WANG JH, 1968, V161, P328, witzel h, 1962, v7, p295, WYCKOFF HW, 1967, V242, P3984 |
| Abstract | Fifty years ago, the group of Tony Mathias and Bob Rabin at University College London deduced the first mechanism for catalysis by an enzyme, ribonuclease [Findlay, D., Herries, D. G., Mathias, A. P., Rabin, B. R., and Ross, C. A. (1961) Nature 190, 781-784]. Here, we celebrate this historic accomplishment by surveying knowledge of enzymology and protein science at that time, facts that led to the formulation of the mechanism, criticisms and alternative mechanisms, data that supported the proposed mechanism, and some of the refinements that have since provided a more precise picture of catalysis of RNA cleavage by ribonucleases. The Mathias and Rabin mechanism has appeared in numerous textbooks, monographs, and reviews and continues to have a profound impact on biochemistry.
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| Short Title | Biochemistry |
