Varian User's Guide (VUG)

Users of this document should use it as it is intended:   as a guide only.
Please do not assume 100% accuracy, since it is not there (and let me know when you find mistakes!!).

I, the Chemistry Department and the University will not accept responsibility for any problems arising from the inadvertent inaccuracies and mistakes that are contained in this User Guide.

This User Guide may be distributed freely with proper acknowledgements. It may not be used for any commercial activities.

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Introduction to the Use of Hermes (Varian Mercury-300) (pdf)

VUG Table of Contents

[Links to Step-by-Step Instructions are provided in section 5 below]

1. Title page, Detailed Table of Contents, and
   Facility Description
    
2. Quick Guide for New Users
   Login; Setup; Lock-n-Shim; Probe Tuning; Acquisition;
   Saving and Deleting Data, and Backups; Logging Out; Don'ts
    
3. Introduction to Unix and VNMR
   Common Unix Commands
   Common VNMR Commands, Parameters, and Flags
   VNMR Directory Structure
   Common Pulse Sequence Setup in VNMR
   VNMR to Bruker-AM/AC Parameter Conversion Table
   Trouble-Shooting
    
4. Operation of Varian 500s
   Proper Exiting and Login In and Initial VNMR Setup
   Commands for First Time and Novice Users
   Probe Changes
   Probe Tuning
   Lock and Shim
    
5. Experiments on Varian Spectrometers
• Normal 1d 1H Acquisition (step-by-step--sbs--instructions)
  1H pw90 Calibration (sbs)
  1H T1 estimates (sbs)
  1d Data Workup and Plotting
   
• X Acquisition (e.g., 31P or 13C)   (sbs)
  Table 6: common Decoupler Parameter Settings;
  13C (X) pw90 and {1H} Decoupler Calibrations
• DEPT   (sbs)
• INEPT   (sbs)
   
• COSY - absolute value fast-COSY   (Chem637 notes)
  2d Data Workup and Plotting
• DQ-COSY   (Chem637 notes)
• TOCSY-1D Spectroscopy (sbs only)
• TOCSY and Other COSY Variants   (Chem637 notes)
• Notes on TOCSY and ROESY (fall 2005)
   
• NOESY-1D Spectroscopy (sbs only)
• 1d NOE-Difference Spectroscopy and HOMODEC   (Chem637 notes)
• NOESY
• ROESY
   
• HETCOR
• HMQC and HSQC   (Chem637 notes including new HSQCAD & gHSQCAD)
• gHMBC + Other Hetero-Correlation Exps   (Chem637 notes including new gHMBCAD)
   
• Presaturation of Solvent Peaks (Chem637 notes)
   
• Deuterium NMR on Varian Spectrometers
• Perfroming 1H{19F} or 19F{1H} Experiments on the INOVA-500
• 205Tl setup on INOVA-500
   
• Measurement of Spin-Lattice Relaxtion Time, T1
• Variable Temperature Experiments on Varian Spectrometers
• Acquiring Kinetics Datasets in VNMR
  For kinetics processing, see also "Notes on Performing Quantitative Analysis Using VNMR" (item 10 below)
   
6. PFG Shimming
    
7. Plotting VNMR Data:
   VNMR plotting - common commands
   VNMR plotting - common parameters; graphical layout
   Porting VNMR Data to PCs/Macs - generating postscript files, and extracting arrayed spectra for NUTS
    
8. Absolute Referencing in VNMR
    
9. Deconvolution Peak Fitting in VNMR
    
10. Notes on Performing Quantitative Analysis Using VNMR
     
11. Notes on Using BioPack in vnmr6.1c