This facility has seven NMR and one ESR spectrometers located in the Paul Bender Chemistry Instrument Center (CIC). Being primarily a student-run operation, service work is typically not provided. Access is obtained via training and checkouts, provided by the facility staff. Detailed Users Guides are available to assist in the use of all UWChemMRF equipment. 

• nmrhfsnap.chem.wisc.edu was replaced on 25 Apr 2008 with ftp://apollo.chem.wisc.edu, which provides access to hermes and high-field NMR data. Any ftp software can be used to access the device, but two possibilities work nicely: (1) On Windows PCs, open Windows Explorer (not Internet Explorer, rather the file manager) and type in the address given above. The device will connect and look very much like any other disk drive. (2) Firefox is a good choice for PCs and Macs, but you'll have to install an add-on called FireFTP. After the installation, enter the address above into Firefox. Right-click (Windows) or Cntl-click (Mac) on a folder and select "Open Link in FireFTP". The add-on works similar to the WS_FTP program provided on the facility PCs.
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• Researchers should always check the integrity of their samples prior to running variable temperature experiments. This criteria is particularly important for sealed samples to be run at elevated temperatures; if pressures are generated that explode the sample in the NMR probe (as recently happened to the INOVA-500 hcx probe), a very expensive repair will result. Testing the sealed samples in a water or oil bath outside the NMR lab is strongly recommended for such situations. Thick-walled tubes can assist with sample integrity, but in general, samples where pressures might rise above 5 atmospheres should not be in the standard setup in the facility.
   
• Referencing 1D X-nucleus and 2D 1H-X spectra is greatly simplified by the use of the Unified Scale. Facility VNMR macros (e.g., xref and decref) make the referencing simple to apply. NUTS macros can be used to reference Bruker 1D data, e.g., from Athena for 31P, 19F or 13C data. See the facility notes, and the AcornNMR website for details.
     
• Use of Shegemi tubes is highly recommended for 13C work, as well as for any other study where sensitivity and experiment time are issues. Shegemi tubes, although considerably more expensive than standard NMR tubes (costing ~$85), increase soluble samples by 2.5x in concentration, saving ~8x in experiment time. We have many examples where an overnight 13C acquisition on the 300s was bettered in 1h on a 500 using a Shegemi tube. Even on the 300s, a poor-quality 4h 13C run can be bettered in 1h by use of a Shegemi tube. See the User Guides -> Other -> Solvents... and -> Sensitivity... for more information.